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• Quercus woodlands are key components of California’s wild landscapes, yet little is known about ectomycorrhizal (EM) fungi in these ecosystems.• We examined the EM community associated with Quercus douglasii using sporocarp surveys and by pooling EM roots and subjecting them to DNA extraction, polymerase chain reaction (PCR), cloning, restriction fragment length polymorphism (RFLP) screening and DNA sequencing.