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Cellular microarrays have become extremely useful in expediting the investigation of large libraries of (bio) materials for both in vitro and in vivo biomedical applications. We have developed an exceedingly simple strategy for the fabrication of non-cell-adhesive substrates supporting the immobilization of diverse (bio) material features, including both monomeric and polymeric adhesion molecules (eg RGD and polylysine), hydrogels, and polymers.