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This work investigated the phytochemical constituents and synergistic antibacterial activity of ethanolic extracts of Mondia whytei roots and Physalis peruviana leaves.

This study employed both qualitative and quantitative methods.

All research works were performed at the Central Science Laboratories, Faculty of science, Kyambogo University between September 2021 and March 2022.

Standard methods were followed for phytochemical screening of ethanolic extracts of Mondia whytei roots, and Physalis peruviana leaves. The potential synergistic antibacterial activity of ethanolic extracts were evaluated through agar well diffusion assay. The bacteria strains were cultured on Mueller-Hinton agar. Sterile cork borer (6 mm diameter) was used to create the wells on petri discs and filled aseptically with 10 µl of the test extract (100 mg/ml) and Chloramphenicol (0.5 mg/ml) was used as the positive control. The interaction between the extracts and the combined extract was assessed by determining their fractional inhibitory concentration (FIC) index.

Alkaloids, flavonoids, tannins, saponins, phenols were found to be present in both extracts. Cardiac glycosides, terpenoids, anthraquinnones and steroids were present only in ethanolic extract of Physalis peruviana leaves. The antibacterial activities of Mondia whytei roots extract, Physalis peruviana leaves extract and combined extract were obtained with the following zone of inhibitions; 19.20 ± 0.72 mm, 19.77 ± 0.60 mm, 25.93 ± 0.83 mm for S. aureus and 15.50 ± 0.46 mm, 15.00 ± 0.26 mm, 17.87 ± 0.61 mm for E.coli respectively …