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As well as systemic vascular endothelial cells, the liver has specialised sinusoidal endothelial cells (LSEC). LSEC dysfunction has been documented in many diseased states yet their phenotype in normal human liver has not been comprehensively assessed. Our aim was to improve characterisation of subsets of endothelial cells and associated pericytes in the human liver. Immunofluorescence microscopy was performed on normal human liver tissue samples to assess endothelial and structural proteins in a minimum of three donors. LSEC are distributed in an acinar pattern and universally express CD36, but two distinctive subsets of LSEC can be identified in different acinar zones. Type 1 LSEC are CD36^hiCD32⁻CD14⁻LYVE-1⁻ and are located in acinar zone 1 of the lobule, while Type 2 LSEC are LYVE-1⁺CD32^hiCD14⁺CD54⁺CD36^mid-lo and are located in acinar zones 2 and 3 of the lobule. Portal tracts and …