作者
Elias Hage, Gavin S Wilkie, Silvia Linnenweber-Held, Akshay Dhingra, Nicolás M Suárez, Julius J Schmidt, Penelope C Kay-Fedorov, Eva Mischak-Weissinger, Albert Heim, Anke Schwarz, Thomas F Schulz, Andrew J Davison, Tina Ganzenmueller
发表日期
2017/6/1
期刊
The Journal of infectious diseases
卷号
215
期号
11
页码范围
1673-1683
出版商
Oxford University Press
简介
Background
Advances in next-generation sequencing (NGS) technologies allow comprehensive studies of genetic diversity over the entire genome of human cytomegalovirus (HCMV), a significant pathogen for immunocompromised individuals.
Methods
Next-generation sequencing was performed on target enriched sequence libraries prepared directly from a variety of clinical specimens (blood, urine, breast milk, respiratory samples, biopsies, and vitreous humor) obtained longitudinally or from different anatomical compartments from 20 HCMV-infected patients (renal transplant recipients, stem cell transplant recipients, and congenitally infected children).
Results
De novo–assembled HCMV genome sequences were obtained for 57 of 68 sequenced samples. Analysis of longitudinal or compartmental HCMV diversity revealed various patterns: no major …
学术搜索中的文章
E Hage, GS Wilkie, S Linnenweber-Held, A Dhingra… - The Journal of infectious diseases, 2017