作者
Kerstin Klein, Pawel A Kabala, Aleksander M Grabiec, Renate E Gay, Christoph Kolling, Lih-Ling Lin, Steffen Gay, Paul P Tak, Rab K Prinjha, Caroline Ospelt, Kris A Reedquist
发表日期
2016/2/1
期刊
Annals of the rheumatic diseases
卷号
75
期号
2
页码范围
422-429
出版商
BMJ Publishing Group Ltd
简介
Objective
To investigate the effects of BET bromodomain protein inhibition on inflammatory activation and functional properties of rheumatoid arthritis synovial fibroblasts (RASF).
Methods
The expression of the BET bromodomain proteins BRD2, BRD3 and BRD4 was analysed in synovial tissue by immunohistochemistry. RASF were stimulated with tumour necrosis factor (TNF)-α, interleukin (IL)-1β and toll-like receptor (TLR) ligands (Pam3, pIC and lipopolysaccharide (LPS)) in the presence or absence of the BET inhibitor I-BET151, or siRNA targeting BRD2, BRD3 and BRD4. RASF expression of inflammatory mediators, including MMP1, MMP3, IL-6 and IL-8, was measured by q-PCR, q-PCR array and ELISA. Cellular viability, apoptosis, proliferation and chemoattractive properties of RASF were investigated using MTT, cell apoptosis ELISA, BrdU-based proliferation and transwell migration assays.
Results
BRD2 …
学术搜索中的文章
K Klein, PA Kabala, AM Grabiec, RE Gay, C Kolling… - Annals of the rheumatic diseases, 2016