作者
Lukasz J Bugaj, Atri T Choksi, Colin K Mesuda, Ravi S Kane, David V Schaffer
发表日期
2013/3
期刊
Nature methods
卷号
10
期号
3
页码范围
249-252
出版商
Nature Publishing Group US
简介
We report an optogenetic method based on Arabidopsis thaliana cryptochrome 2 for rapid and reversible protein oligomerization in response to blue light. We demonstrated its utility by photoactivating the β-catenin pathway, achieving a transcriptional response higher than that obtained with the natural ligand Wnt3a. We also demonstrated the modularity of this approach by photoactivating RhoA with high spatiotemporal resolution, thereby suggesting a previously unknown mode of activation for this Rho GTPase.
学术搜索中的文章
LJ Bugaj, AT Choksi, CK Mesuda, RS Kane… - Nature methods, 2013