作者
A Anand, P Vora, S Mahajan, V Trivedi, V Chhaniwal, A Singh, R Leitgeb, B Javidi
发表日期
2014/1
期刊
Pramana
卷号
82
页码范围
71-78
出版商
Springer India
简介
Microscopy using visible electromagnetic radiation can be used to investigate living cells in various environments. But bright field microscopy only provides two-dimensional (2D) intensity distribution at a single object plane. One of the ways to retrieve object height/thickness information is to employ quantitative phase microscopic (QPM) techniques. Interferometric QPM techniques are widely used for this. Digital holographic microscopy (DHM) is one of the state-of-the-art methods for quantitative three-dimensional (3D) imaging. Usually it is implemented in two-beam geometry, which is prone to mechanical vibrations. But to study dynamics of objects like red blood cells, one needs temporal stability much better than the fluctuations of the object, which the two-beam geometry fails to deliver. One way to overcome this hurdle is to use self-referencing techniques, in which a portion of the object beam will act as …
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