作者
Angeliki Karapanagioti, Narjes Nasiri-Ansari, Athanasios Moustogiannis, George C Trigas, Georgios Zografos, Chrysanthi Aggeli, Georgios Kyriakopoulos, Theodosia Choreftaki, Anastassios Philippou, Gregory Kaltsas, Eva Kassi, Anna Angelousi
发表日期
2023/8
期刊
Endocrine
卷号
81
期号
2
页码范围
357-367
出版商
Springer US
简介
Purpose
CHCHD2 is an antiapoptotic mitochondrial protein acting through the BCL2/BAX pathway in various cancers. However, data on the regulatory role of CHCHD2 in adrenal tumourigenesis are scarce.
Methods
We studied the expression of CHCHD2, BCL2, and BAX in human adrenocortical tissues and SW13 cells. mRNA and protein levels were analyzed through qPCR and immunoblotting, respectively, in 16 benign adrenocortical neoplasms (BANs), along with their adjacent normal adrenal tissues (controls), and 10 adrenocortical carcinomas (ACCs). BCL2/BAX mRNA expression was also analyzed in SW13 cells after CHCHD2 silencing. MTS, flow cytometry and scratch assays were performed to assess cell viability, apoptosis, and invasion, respectively.
Results
BCL2 and CHCHCD2 mRNA and protein expression was increased in BANs compared to normal adrenal tissues whereas BAX was decreased …
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A Karapanagioti, N Nasiri-Ansari, A Moustogiannis… - Endocrine, 2023