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Thailand is situated in the region of high Bambusa biodiversity which extends from southern China to the Indochinese Peninsula. However, our understanding of Bambusa diversity and biogeography in this region remains incomplete, due to the lack of both local expertise and diagnostic characters on specimens, especially reproductive parts, which are relatively rare occurrences in the wild. Ongoing botanical surveys and flora projects in this region have led to the discovery of new Bambusa species in recent years. Here, a new species, B. lituiformis, and the first record from Thailand of B. nghiana, a Vietnamese species, are reported. The new species and record are described and illustrated from complete collections, using both vegetative and reproductive parts.