作者
Abhilasha Sinha, Sumit Agarwal, Deepak Parashar, Archana Verma, Shikha Saini, Nirmala Jagadish, Abdul S Ansari, Nirmal K Lohiya, Anil Suri
发表日期
2013/12
期刊
Journal of Experimental & Clinical Cancer Research
卷号
32
页码范围
1-11
出版商
BioMed Central
简介
Background
Recently, we reported an association of a novel cancer testis (CT) antigen, sperm-associated antigen 9 (SPAG9) expression in breast cancer clinical samples, indicating its potential role in carcinogenesis. Around 15% breast cancers are designated as triple-negative for which treatment modalities are limited. Therefore, in the present study, we assessed the role of SPAG9 in triple-negative breast cancer cells.
Methods
SPAG9 mRNA and protein expression was investigated in various breast cancer cells of different hormone receptor status and different subtypes by employing reverse transcriptase-polymerase chain reaction (RT-PCR), real time PCR, Western blotting, indirect immunofluorescence (IIF) and fluorescence activated cell sorting (FACS). Employing plasmid-based small interfering RNA (siRNA) approach, knockdown of SPAG9 was …
学术搜索中的文章
A Sinha, S Agarwal, D Parashar, A Verma, S Saini… - Journal of Experimental & Clinical Cancer Research, 2013