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4. Results

The new primers were evaluated with RNA from a panel of stool samples previously used for comparison of the diagnostic assays employed in different European laboratories (Vinjé et al., submitted). The panel consisted of 20 stool samples of diverse genotypes from each of four European laboratories involved in a EU-sponsored project (QLK1-1999-00594), three negative controls and nine stool samples of selected genotypes from our own collection. The latter were used for a sensitivity estimation by dilution of RNR (Vinjé et al., submitted). The modified primers JV12Y/JV13I detected NLV in 60 of 91 panel samples, seven more than detection with JV12/JV13. In particular additional viruses of the Leeds genotype were detected. To determine the detection limit for JV12Y/JV13I we tested several dilutions of isolated RNA dilution ranges previously used for sensitivity estimation. This showed that for seven of …