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This paper reports the application of near-infrared Raman spectroscopy to detect ephedrine in biological medium. At present time, the doping control for ephedrine in athletes uses the urinalysis by the gas chromatography/mass spectrometry, with main disadvantage the need of collecting urine and the time delay to obtain results. This work aims to develop a noninvasive technique that will allow to evaluate the concentration of the ephedrine in a real time diagnosis. A Raman system composed by a Ti: Saphire laser pumped by an Argon laser was used, operating at the wavelength of 785 nm, with a laser power of 70 mW at sample position. Raman scattered photons were collected by af/l. 8 spectrometer and a N 2-cooled CCD detector. Ephedrine Raman peaks at 1002 and 1603 cm− 1 were studied, opening possibility for the identification and quantification of ephedrine. Raman spectra of ephedrine with different …