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Biologically synthesized nanoparticles have been widely using in the field of medicine. Biogenesis of nanoparticles have proven to be better methods due to slower kinetics and they offer better manipulation and control over crystal growth and their stabilization. The present study revealed that the phytosynthesis of silver nanoparticles from 1 mM AgNO3 solution through the leaf extract of Svensonia hyderabadensis as reducing agent as well as capping agent. Nanoparticles were characterized using UV-Vis Spectrophotometry, XRD and SEM. X-ray diffraction and SEM analysis showed the average particle size of 45 nm with spherical shape. Further these biologically synthesized nanoparticles were found to be inhibit against different microorganisms. Extracellular synthesis of nanoparticles using dried leaf powder of Svensonia hyderabadensis is conventional, eco-friendly and cost effective.