作者
David S Miller, Stephanie N Nobmann, Heike Gutmann, Michael Toeroek, Juergen Drewe, Gert Fricker
发表日期
2000/12/1
期刊
Molecular pharmacology
卷号
58
期号
6
页码范围
1357-1367
出版商
American Society for Pharmacology and Experimental Therapeutics
简介
To identify specific transporters that drive xenobiotics from central nervous system to blood, the accumulation of fluorescent drugs was studied in isolated capillaries from rat and pig brain using confocal microscopy and quantitative image analysis. Luminal accumulation of daunomycin and of fluorescent derivatives of cyclosporine A (CSA) and ivermectin was concentrative, specific, and energy-dependent (inhibition by NaCN). Transport was reduced by PSC 833, ivermectin, verapamil, CSA, and vanadate, but not by leukotriene C4(LTC4), indicating the involvement of P-glycoprotein. Luminal accumulation of the fluorescent organic anions sulforhodamine 101 and fluorescein methotrexate was also concentrative, specific, and energy-dependent. LTC4, chlorodinitrobenzene, and vanadate reduced transport of these compounds, but PSC 833 and verapamil did not, indicating the involvement of a multidrug resistance …
引用总数
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DS Miller, SN Nobmann, H Gutmann, M Toeroek… - Molecular pharmacology, 2000