作者
Jun Ge, Diannan Lu, Jun Wang, Ming Yan, Yunfeng Lu, Zheng Liu
发表日期
2008/11/13
期刊
The Journal of Physical Chemistry B
卷号
112
期号
45
页码范围
14319-14324
出版商
American Chemical Society
简介
The assembly of a monomer around an enzyme as the essential step in the fabrication of enzyme nanogel by in situ polymerization was illustrated by molecular dynamics simulation and evidenced by a fluorescence resonance energy transfer spectrum, using lipase/acrylamide as a model system. The subsequent polymerization generated a hydrophilic gel network which not only strengthened the protein structural integrity via multipoint linkage but also increased the number of intramolecular H-bonds of the encapsulated protein, as suggested by the blue shift of the fluorescence spectrum of the encapsulated lipase. This greatly enhanced the stability of lipase at high temperature, as experimentally demonstrated. The exclusion of polar solvent molecules from the encapsulated enzyme, in contrast to the enrichment of water molecules, due to the presence of a hydrophilic gel network was displayed. This established a …
学术搜索中的文章
J Ge, D Lu, J Wang, M Yan, Y Lu, Z Liu - The Journal of Physical Chemistry B, 2008