作者
Lian Li, Quan Jiang, Guangliang Ding, Li Zhang, Zheng Gang Zhang, Qingjiang Li, Swayamprava Panda, Mei Lu, James R Ewing, Michael Chopp
发表日期
2010/3
期刊
Journal of Cerebral Blood Flow & Metabolism
卷号
30
期号
3
页码范围
653-662
出版商
SAGE Publications
简介
We tested the hypotheses that administration routes affect the migration and distribution of grafted neural progenitor cells (NPCs) in the ischemic brain and that the ischemic lesion plays a role in mediating the grafting process. Male Wistar rats (n=41) were subjected to 2-h middle cerebral artery occlusion (MCAo), followed 1 day later by administration of magnetically labeled NPCs. Rats with MCAo were assigned to one of three treatment groups targeted for cell transplantation intra-arterially (IA), intracisternally (IC), or intravenously (IV). MRI measurements consisting of T2-weighted imaging and three-dimensional (3D) gradient echo imaging were performed 24 h after MCAo, 4 h after cell injection, and once a day for 4 days. Prussian blue staining was used to identify the labeled cells, 3D MRI to detect cell migration and distribution, and T2 map to assess lesion volumes. Intra-arterial (IA) administration showed …
学术搜索中的文章
L Li, Q Jiang, G Ding, L Zhang, ZG Zhang, Q Li… - Journal of Cerebral Blood Flow & Metabolism, 2010