作者
Rui Lan Zhang, Michael Chopp, Zheng G Zhang, Quan Jiang, James R Ewing
发表日期
1997/8/22
期刊
Brain research
卷号
766
期号
1-2
页码范围
83-92
出版商
Elsevier
简介
We developed a new model of embolic cerebral ischemia in the rat which provides a reproducible and predictable infarct volume within the territory supplied by the middle cerebral artery (MCA). The MCA was occluded by an embolus in Wistar rats (n=71). An additional three non-embolized rats were used as a control. Cerebral blood flow (CBF) was measured by means of laser Doppler flowmetry (LDF) and perfusion weighted imaging (PWI) before and after embolization. The evolution of the lesion was monitored by diffusion weighted imaging (DWI). Cerebral vascular perfusion patterns were examined using laser scanning confocal microscopy. Infarct volumes were measured on hematoxylin and eosin (H&E) stained coronal sections. The lodgment of the clot at the origin of the MCA and the ischemic cell damage were examined using light microscopy. Regional CBF in the ipsilateral parietal cortex decreased to 43 …
引用总数
学术搜索中的文章
RL Zhang, M Chopp, ZG Zhang, Q Jiang, JR Ewing - Brain research, 1997