作者
Ying Yang, Fan Shen, Wei Huang, Shanshan Qin, Jing-Tao Huang, Consolato Sergi, Bi-Feng Yuan, Song-Mei Liu
发表日期
2019/3
期刊
The Journal of Clinical Endocrinology & Metabolism
卷号
104
期号
3
页码范围
665-673
出版商
Endocrine Society
简介
Context
N 6-methyladenosine (m6A) in mRNA is the most abundant and reversible modification. However, the mechanism behind the decrease in m6A in patients with type 2 diabetes (T2D) has not yet been thoroughly investigated.
Objective
To clarify whether glucose is involved in the dynamic regulation of m6A in T2D and to identify a possible underlying mechanism.
Methods
Liquid chromatography/electrospray ionization/tandem mass spectrometry and quantitative PCR were performed to determine the m6A content and the mRNA expression of target genes in 102 patients with T2D and 107 controls. An additional 12 patients with normal fasting blood glucose, emergency hyperglycemia, or emergency hypoglycemia, as well as HepG2 cells with high-glucose treatment and FTO knockout or overexpression were used to confirm the initial …
学术搜索中的文章
Y Yang, F Shen, W Huang, S Qin, JT Huang, C Sergi… - The Journal of Clinical Endocrinology & Metabolism, 2019