作者
Matthias Merker, Thomas A Kohl, Ivan Barilar, Sönke Andres, Philip W Fowler, Erja Chryssanthou, Kristian Ängeby, Pontus Jureen, Danesh Moradigaravand, Julian Parkhill, Sharon J Peacock, Thomas Schön, Florian P Maurer, Timothy Walker, Claudio Köser, Stefan Niemann
发表日期
2020/12
期刊
Genome Medicine
卷号
12
页码范围
1-8
出版商
BioMed Central
简介
Background
A comprehensive understanding of the pre-existing genetic variation in genes associated with antibiotic resistance in the Mycobacterium tuberculosis complex (MTBC) is needed to accurately interpret whole-genome sequencing data for genotypic drug susceptibility testing (DST).
Methods
We investigated mutations in 92 genes implicated in resistance to 21 anti-tuberculosis drugs using the genomes of 405 phylogenetically diverse MTBC strains. The role of phylogenetically informative mutations was assessed by routine phenotypic DST data for the first-line drugs isoniazid, rifampicin, ethambutol, and pyrazinamide from a separate collection of over 7000 clinical strains. Selected mutations/strains were further investigated by minimum inhibitory concentration (MIC) testing.
Results
Out of 547 phylogenetically …
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M Merker, TA Kohl, I Barilar, S Andres, PW Fowler… - Genome Medicine, 2020