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A method is described for the synthesis of a nanocomposite containing FeOOH and N-doped carbon nanosheets. The nanocomposite was synthesized by a hydrothermal method using a Fe₃O₄/chitosan nanocomposite as the precursor. The nanocomposite displays peroxidase-like activity and catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H₂O₂. This results in the formation of a blue colored product with an absorption maximum at 652 nm in the UV-vis spectra. Based on these findings, colorimetric assays were worked out for both hydrogen peroxide and glucose. The H₂O₂ assay works in the 5 to 19 μM concentration range, and the limit of detection is 5 nM. The glucose assay works in the 8 μM to 0.8 mM concentration range and has a 0.2 μM detection limit. The method was successfully applied to the determination of glucose in human urine …