查看文章

This study examined the contributions of native gut probiotic bacteria in production of rice bran microbial metabolites with bioactivity and nutritional importance. The study hypothesize that bacteria and yeast probiotics utilize distinct genes for bidirectional influence on bacterial cross feeding and host bioavailability of rice bran vitamins, while decreasing undesirable molecules.

A one/two step fermentation protocol was applied to rice bran (RBT-300) with the following strains Lactobacillus fermentum ATCC 23271, L. paracasei ATCC 21052_R1, L. rhamnosus GG, Escherichia coli Nissle 1917, and Bifidobacterium longum ATCC 55813 before and after incubation with yeast probiotic Saccharomyces boulardii. Metabolomic analysis was performed following 80% methanol-water under vigorous shaking (2 min) followed by centrifugation to remove proteins. Extracts were applied to reverse phase (RP …