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The Italian agri-food sector has traditionally been one of the strongest in the national socio-economic system. In the last ten years, commercial exchange trends have shown growing openness towards foreign countries and in particular to European Union (EU) countries. Both the primary sector and food industry are strongly influenced by their territorial location so much so that several authors have highlighted territorial specialisation and its effects on that territory’s features also considering the contribution of the Italian Regions. The following work will analyse the patterns of agri-food specialisation in the Italian Regions. In particular, the Lafay Index will be used to evaluate competitive advantage at a Regional level.