查看文章

Protocol for rapid in vitro propagation of Ceropegia candelabrum L. (Asclepiadaceae) through axillary bud multiplication was established. Murashige and Skoog (MS) medium with 8.87 μM N ⁶-benzyladenine (BA) and 2.46 μM indole-3-butyric acid (IBA) was best suited for axillary bud proliferation inducing a mean of eight shoots / node. Excision and culture of the nodal segments from the in vitro shoots on fresh medium with same concentrations of BA and IBA facilitated development of more than 10 shoots / node. Subsequent cultures enhanced the rate of shoot proliferation. Shoots developed were rooted best on half strength MS with 0.49 μM IBA. Starting from a single node explant, 250 rooted shoots were obtained within 120 days. Plantlets established in pots exhibited 90% survival. Plantlets successfully established in field exhibited morphological characters identical to mother plants.