作者
Alexander J Ninfa, E Gottlin Ninfa, Andrei N Lupas, Ann Stock, Boris Magasanik, Jeff Stock
发表日期
1988/8
期刊
Proceedings of the National Academy of Sciences
卷号
85
期号
15
页码范围
5492-5496
简介
We demonstrate by using purified bacterial components that the protein kinases that regulate chemotaxis and transcription of nitrogen-regulated genes, CheA and NRII, respectively, have cross-specificities: CheA can phosphorylate the Ntr transcription factor NRI and thereby activate transcription from the nitrogen-regulated glnA promoter, and NRII can phosphorylate CheY. In addition, we find that a high intracellular concentration of a highly active mutant form of NRII can suppress the smooth-swimming phenotype of a cheA mutant. These results argue strongly that sensory transduction in the Ntr and Che systems involves a common protein phosphotransfer mechanism.
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AJ Ninfa, EG Ninfa, AN Lupas, A Stock, B Magasanik… - Proceedings of the National Academy of Sciences, 1988