作者
R Garand, K Beldjord, H Cavé, C Fossat, I Arnoux, V Asnafi, Y Bertrand, ML Boulland, C Brouzes, E Clappier, E Delabesse, T Fest, F Garnache-Ottou, F Huguet, MC Jacob, E Kuhlein, S Marty-Grès, A Plesa, N Robillard, M Roussel, J Tkaczuk, H Dombret, E Macintyre, N Ifrah, MC Béné, A Baruchel
发表日期
2013/2
期刊
Leukemia
卷号
27
期号
2
页码范围
370-376
出版商
Nature Publishing Group
简介
Minimal residual disease (MRD) quantification is widely used for therapeutic stratification in pediatric acute lymphoblastic leukemia (ALL). A robust, reproducible, sensitivity of at least 0.01% has been achieved for IG/TCR clonal rearrangements using allele-specific quantitative PCR (IG/TCR-QPCR) within the EuroMRD consortium. Whether multiparameter flow cytometry (MFC) can reach such inter-center performance in ALL MRD monitoring remains unclear. In a multicenter study, MRD was measured prospectively on 598 follow-up bone marrow samples from 102 high-risk children and 136 adult ALL patients, using IG/TCR-QPCR and 4/5 color MFC. At diagnosis, all 238 patients (100%) had at least one suitable MRD marker with 0.01% sensitivity, including 205/238 samples (86%) by using IG/TCR-QPCR and 223/238 samples (94%) by using MFC. QPCR and MFC were evaluable in 495/598 (83%) samples …
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R Garand, K Beldjord, H Cavé, C Fossat, I Arnoux… - Leukemia, 2013