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Breeding projects aimed at increasing starch amylose would benefit by having a rapid starch extraction method requiring only non‐hazardous reagents and leaving the native granule intact for functional analyses. A study was, therefore, designed to investigate the use of a neutral protease for the removal of protein during the starch extraction process from the grain of high‐amylose corn. Sets of F2 ears presumed to be homozygous for the recessive amylose‐extender (ae) allele and segregating high amylose modifier gene(s) were used in the study and ranged in amylose content from 55% to around 70%, although several non‐mutant genotypes (˜25%) occurred because of visual misclassification of the ae kernels. Starches from samples were all initially extracted by grinding and filtering, then further treated in three ways including either 1) no protein removal, 2) toluene and saline washes or 3) use of neutral …