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An isolate (87) of the yeast Pichia guilliermondii, protects apples from postharvest fruit rotting fungi Botrytis cinerea and Penicillium expansum. In order to examine the yeast-pathogen interaction, B. cinerea was grown on agar plates overlayed with cellophane. Effective and non-effective yeast isolates were applied near the young hyphal growth. Samples were taken 24 h later from the area where the fungi and yeast had intersected. Light microscopy revealed a general attachment of the effective biocontrol agent P. guilliermondii (isolate 87) and a non-effective isolate (117) of Debaryomyces hansenii. Low temperature scanning electron microscopy (LTSEM) indicated that both species of yeast attached to the fungal hyphae, but the 87 isolate attached fastidiously. Twenty-four hours after applying the 87 isolate to B. cinerea, pitting and collapse of the hyphae were observed. These observations were confirmed using …