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The aim of the current study was to indicate the biochemical changes during the preparation and competitive soccer period. The literature review showed that biochemical parameters such as transaminases, enzymes and other factors change significantly from the preparation to the competitive period. The sample of the study included twenty five professional soccer players whose biochemical parameters assessed in the beginning of the preparation period, in the middle, in the end of the preparation period, as well as in the middle of the competitive period. The parameters that were assessed were SGPT, SGOT, ?-GT, CPK, LDH, calcium, potassium, magnesium, sodium, creatinine, triglycerides, urea, uric acid, creatinine and B-12. The results showed that SGPT, SGOT and CPK reduced during the preparation period whereas they increased in the competitive period. Furthermore, LDH, urea, uric acid and creatinine reduced from the beginning of the preparation period to the competitive period. Calcium also reduced during the competitive period whereas magnesium, sodium and B-12 increased. These changes are probably explained by the training aim to improve the aerobic and anaerobic capacity of the players throughout the training sessions of the preparation period.