作者
Feng Zhang, Aurore Labourel, Mireille Haon, Minna Kemppainen, Emilie Da Silva Machado, Nicolas Brouilly, Claire Veneault‐Fourrey, Annegret Kohler, Marie‐Noëlle Rosso, Alejandro Pardo, Bernard Henrissat, Jean‐Guy Berrin, Francis Martin
发表日期
2022/3
期刊
New Phytologist
卷号
233
期号
6
页码范围
2534-2547
简介
- In ectomycorrhiza, root penetration and colonization of the intercellular space by symbiotic hyphae is thought to rely on the mechanical force that results from hyphal tip growth, enhanced by the activity of secreted cell‐wall‐degrading enzymes.
- Here, we characterize the biochemical properties of the symbiosis‐induced polygalacturonase LbGH28A from the ectomycorrhizal fungus Laccaria bicolor. The transcriptional regulation of LbGH28A was measured by quantitative PCR (qPCR). The biological relevance of LbGH28A was confirmed by generating RNA interference (RNAi)‐silenced LbGH28A mutants. We localized the LbGH28A protein by immunofluorescence confocal and immunogold cytochemical microscopy in poplar ectomycorrhizal roots.
- Quantitative PCR confirmed the induced expression of LbGH28A during ectomycorrhiza formation. Laccaria bicolor RNAi mutants have a lower ability to establish …
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F Zhang, A Labourel, M Haon, M Kemppainen… - New Phytologist, 2022