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Ten duplicate samples of fresh meat (beef) were randomly sampled from 2 major markets (Watt and Marian) in Calabar, Nigeria and analyzed microbiologically for the rates of Gram negative bacteria. The mean microbial load on the fresh meat from Watt market ranged between 2.62× 104 4.84 x 104 cfu/g and total coliform count between 1.05× 103 3.72× 103 cfu/g while the fresh meat from Marian market ranged between 2.24× 104 5.01× 104 cfu/g and total coliform count between 1.23 x 103 3.42× 103cfu/g. A total of 36 isolates belonging to eight genera include Klebsiella pneumoniae [6 (16.7%)] which was the most predominant, followed by Enterobacter spp [5 (13.9%)], Citrobacter freundii [5 (13.9%)], Pseudomonas aeruginosa [4 (11.1%)], Escherichia coli [4 (11.1%)], Salmonella spp [4 (11.1%)], Serratia marcescens [4 (11.1%)], and Pseudomonas spp [3 (8.3%)]. Proteus vulgaris [1 (2.8%)] was less predominant …