作者
Byron Miyazawa, Alpa Trivedi, Padma Priya Togarrati, Daniel Potter, Gyulnar Baimukanova, Lindsay Vivona, Maximillian Lin, Ernesto Lopez, Rachael Callcut, Amit K Srivastava, Lucy Z Kornblith, Alexander T Fields, Martin A Schreiber, Charles E Wade, John B Holcomb, Shibani Pati
发表日期
2019/6/1
期刊
Journal of Trauma and Acute Care Surgery
卷号
86
期号
6
页码范围
931-942
出版商
LWW
简介
BACKGROUND
Platelet (Plt)-derived extracellular vesicles (Plt-EVs) have hemostatic properties similar to Plts. In addition to hemostasis, Plts also function to stabilize the vasculature and maintain endothelial cell (EC) barrier integrity. We hypothesized that Plt-EVs would inhibit vascular EC permeability, similar to fresh Plts. To investigate this hypothesis, we used in vitro and in vivo models of vascular endothelial compromise and bleeding.
METHODS
In the vitro model, Plt-EVs were isolated by ultracentrifugation and characterized for Plt markers and particle size distribution. Effects of Plts and Plt-EVs on endothelial barrier function were assessed by transendothelial electrical resistance measurements and histological analysis of endothelial junction proteins. Hemostatic potential of Plt-EVs and Plts was assessed by multiple electrode Plt aggregometry. Using an in vivo model, the effects of Plts and Plt-EVs on vascular …
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B Miyazawa, A Trivedi, PP Togarrati, D Potter… - Journal of Trauma and Acute Care Surgery, 2019