作者
Ryuichiro Akagi, Yorikazu Akatsu, Kathleen M Fisch, Oscar Alvarez-Garcia, Takeshi Teramura, Yuta Muramatsu, Masahiko Saito, Takahisa Sasho, Andrew I Su, Martin K Lotz
发表日期
2017/6/1
期刊
Osteoarthritis and cartilage
卷号
25
期号
6
页码范围
943-951
出版商
WB Saunders
简介
Objectives
Circadian rhythm (CR) was identified by RNA sequencing as the most dysregulated pathway in human osteoarthritis (OA) in articular cartilage. This study examined circadian rhythmicity in cultured chondrocytes and the role of the CR genes NR1D1 and BMAL1 in regulating chondrocyte functions.
Methods
RNA was extracted from normal and OA-affected human knee cartilage (n = 14 each). Expression levels of NR1D1 and BMAL1 mRNA and protein were assessed by quantitative PCR and immunohistochemistry. Human chondrocytes were synchronized and harvested at regular intervals to examine circadian rhythmicity in RNA and protein expression. Chondrocytes were treated with small interfering RNA (siRNA) for NR1D1 or BMAL1, followed by RNA sequencing and analysis of the effects on the transforming growth factor beta (TGF-β) pathway.
Results
NR1D1 and BMAL1 mRNA and protein levels …
学术搜索中的文章
R Akagi, Y Akatsu, KM Fisch, O Alvarez-Garcia… - Osteoarthritis and cartilage, 2017
