作者
Kazunori Sugiura, Hideaki Tanaka, Genji Kurisu, Ken-ichi Wakabayashi, Toru Hisabori
发表日期
2019/6/1
期刊
Biochimica et Biophysica Acta (BBA)-General Subjects
卷号
1863
期号
6
页码范围
1098-1107
出版商
Elsevier
简介
Change in the intracellular redox state is a consequence of various metabolic reactions, which simultaneously regulates various physiological phenomena in cells. Monitoring the redox state in living cells is thus very important for understanding cellular physiology. Various genetically encoded fluorescent redox sensors have therefore been developed. Recently, we developed oxidation-sensitive fluorescent proteins named Oba-Q (Sugiura, K., et al. (2015) Biochem. Biophys. Res. Commun. 457, 242–248), which exhibit dramatic quenching under oxidizing conditions. To extend the range of uses of redox sensor proteins, we refined these proteins based on the molecular architecture applied to Oba-Q, and successfully produced several redox sensor proteins based on CFP and YFP. Interestingly, some of these sensor proteins showed the reverse changes in emission compared with Oba-Q, implying remarkable …
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K Sugiura, H Tanaka, G Kurisu, K Wakabayashi… - Biochimica et Biophysica Acta (BBA)-General Subjects, 2019