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1. Na⁺‐dependent phosphate transport activity from bovine brush border membrane vesicles (BBMV) was reconstituted into artificial phospholipid membranes. It was shown, using lectin affinity chromatography, that transport activity was mediated by a glycoprotein.

2. The bovine kidney epithelial cell line NBL‐1 expresses a Na⁺‐dependent phosphate transporter which shows adaptive regulation in response to various media phosphate concentrations. Cells incubated in low‐phosphate medium and medium containing 10 mM phosphate have V_max values of 6.34±0.18 and 2.95 ± 0.1 nmol · mg^‐1 and K_m values of 17.1 ± 3.0 and 36.3 ± 7.14 μM for phosphate transport, respectively. Thus there is a significant (P<0.001) decrease in the K_m and increase in the V_max of transport when cells are transferred from 10 mM phosphate medium to low‐phosphate medium.

3. Adaptation of phosphate transport in NBL‐1 cells to …