作者
Paul M Coen, John J Dubé, Francesca Amati, Maja Stefanovic-Racic, Robert E Ferrell, Frederico GS Toledo, Bret H Goodpaster
发表日期
2010/1/1
期刊
Diabetes
卷号
59
期号
1
页码范围
80-88
出版商
American Diabetes Association
简介
OBJECTIVE
We tested the primary hypotheses that sphingolipid and diacylglycerol (DAG) content is higher within insulin-resistant muscle and that the association between intramyocellular triglycerides (IMTG) and insulin resistance is muscle fiber type specific.
RESEARCH DESIGN AND METHODS
A nested case-control analysis was conducted in 22 obese (BMI >30 kg/m2) women who were classified as insulin-resistant (IR; n = 12) or insulin-sensitive (IS; n = 10), determined by hyperinsulinemic-euglycemic clamp (>30% greater in IS compared with IR, P < 0.01). Sphingolipid and DAG content was determined by high-performance liquid chromatography–tandem mass spectrometry. Fiber type–specific IMTG content was histologically determined. Gene expression was determined by quantitative PCR.
RESULTS
Total (555 ± 53 vs. 293 ± 54 pmol/mg protein, P …
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