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Aim To evaluate knowledge, practices, and attitudes to breastfeeding among Croatian health professionals before and after the United Nations International Children’s Emergency Fund/World Health Organization (UNICEF/WHO) 20-hour course.

Methods Study included 5 of 9 maternity hospitals in southern Croatia, which had completed the UNICEF/WHO 20-hour breastfeeding training course between December 2007 and February 2009. An anonymous questionnaire testing knowledge, practices, and attitudes was distributed to 424 health professionals before training and to 308 health professionals afterwards. Health professionals’ attitudes were assessed using the validated Iowa Infant Feeding Attitude Scale.