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In an attempt to reduce the variability in the yields of human islet isolations and to identify donor factors that were potentially deleterious, we retrospectively reviewed 153 human islet isolations in our center over a 3-year period. Isolations were performed using controlled collagenase perfusion via the duct, automated dissociation, and Ficoll purification. Factors leading to successful isolations (recovery of> 100,000 islet equivalents at a purity> 50%) were analyzed retrospectively using univariate and multivariate analysis. Critical factors in the multiorgan cadaveric donors that were identified using univariate analysis included donor age (P< 0.01), body mass index (BMI)(P< 0.01), cause of death (P< 0.01), and prolonged hypotensive episodes (systolic blood pressure< 90 mmHg or mean arterial pressure< 60 mmHg for> 15 min) requiring high vasopressors (> 15 μg/kg/min dopamine or> 5 μg/kg/min Levophed)(P< 0.01 …