作者
Milad Riazifar, M Rezaa Mohammadi, Egest J Pone, Ashish Yeri, Cecilia Lasser, Aude I Segaliny, Laura L McIntyre, Ganesh Vilas Shelke, Elizabeth Hutchins, Ashley Hamamoto, Erika N Calle, Rossella Crescitelli, Wenbin Liao, Victor Pham, Yanan Yin, Jayapriya Jayaraman, Jonathan RT Lakey, Craig M Walsh, Kendall Van Keuren-Jensen, Jan Lotvall, Weian Zhao
发表日期
2019/5/22
期刊
ACS nano
卷号
13
期号
6
页码范围
6670-6688
出版商
American Chemical Society
简介
To dissect therapeutic mechanisms of transplanted stem cells and develop exosome-based nanotherapeutics in treating autoimmune and neurodegenerative diseases, we assessed the effect of exosomes secreted from human mesenchymal stem cells (MSCs) in treating multiple sclerosis using an experimental autoimmune encephalomyelitis (EAE) mouse model. We found that intravenous administration of exosomes produced by MSCs stimulated by IFNγ (IFNγ-Exo) (i) reduced the mean clinical score of EAE mice compared to PBS control, (ii) reduced demyelination, (iii) decreased neuroinflammation, and (iv) upregulated the number of CD4+CD25+FOXP3+ regulatory T cells (Tregs) within the spinal cords of EAE mice. Co-culture of IFNγ-Exo with activated peripheral blood mononuclear cells (PBMCs) cells in vitro reduced PBMC proliferation and levels of pro-inflammatory Th1 and Th17 cytokines including IL-6, IL …
学术搜索中的文章
M Riazifar, MR Mohammadi, EJ Pone, A Yeri, C Lasser… - ACS nano, 2019