作者
Wenji Li, Doug Pung, Zheng-Yuan Su, Yue Guo, Chengyue Zhang, Anne Yuqing Yang, Xi Zheng, Zhi-Yun Du, Kun Zhang, Ah-Ng Kong
发表日期
2016/4/18
期刊
Chemical research in toxicology
卷号
29
期号
4
页码范围
694-703
出版商
American Chemical Society
简介
It has previously been shown that curcumin can effectively inhibit prostate cancer proliferation and progression in TRAMP mice, potentially acting through the hypomethylation of the Nrf2 gene promoter and hence activation of the Nrf2 pathway to enhance cell antioxidative defense. FN1 is a synthetic curcumin analogue that shows stronger anticancer activity than curcumin in other reports. We aimed to explore the epigenetic modification of FN1 that restores Nrf2 expression in TRAMP-C1 cells. Stably transfected HepG2-C8 cells were used to investigate the effect of FN1 on the Nrf2- antioxidant response element (ARE) pathway. Real-time quantitative PCR and Western blotting were applied to study the influence of FN1 on endogenous Nrf2 and its downstream genes. Bisulfite genomic sequencing (BGS) and methylated DNA immunoprecipitation (MeDIP) were then performed to examine the methylation profile of the …
学术搜索中的文章
W Li, D Pung, ZY Su, Y Guo, C Zhang, AY Yang… - Chemical research in toxicology, 2016