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Microbial volatile organic compounds (mVOCs) and its potentiality in plant growth and development is still an unexplored area. The in vitro study on bipartite plate showed inhibition of Fusarium oxysporum f.sp lycopersici (FOL) by volatiles of Trichoderma asperellum BHU P1 and Ochrobactrum sp. BHU-PB1 over control. The seed germination and seedling growth was recorded maximum in plant exposed to VOCs of Ochrobactrum sp. in both magenta box (in vitro) and pot (in vivo) experiment. The growth parameters as seed germination, shoot length, root length, fresh weight, dry weight, number of lateral roots and number of leaflets was compared to be higher in microbial volatile metabolites treated plants as compared to control. Disease incidence in T. asperellum and Ochrobactrum sp. volatile treated tomato plants were 43.66% and 41.33%, respectively at 20 days post inoculation (dpi) whereas untreated control …