作者
Thijis van de Laar, Oliver Pybus, Sylvia Bruisten, David Brown, Mark Nelson, Sanjay Bhagani, Martin Vogel, Alex Baumgarten, Marie-Laure Chaix, Martin Fisher, Hannelore Gőtz, Gail V Matthews, Stefan Neifer, Peter White, William Rawlinson, Stanislav Pol, Jurgen Rockstroh, Roel Coutinho, Greg J Dore, Geoffrey M Dusheiko, M Danta
发表日期
2009/5/1
期刊
Gastroenterology
卷号
136
期号
5
页码范围
1609-1617
出版商
WB Saunders
简介
BACKGROUND & AIMS
Since 2000, there has been a marked rise in acute hepatitis C virus (HCV) in human immunodeficiency virus (HIV)-positive men who have sex with men (MSM). We conducted an international phylogenetic study to investigate the existence of an HCV transmission network among MSM.
METHODS
HIV-positive MSM diagnosed with recent HCV (n = 226) in England (107), The Netherlands (58), France (12), Germany (25), and Australia (24) between 2000 and 2006 were enrolled into a molecular phylogenetic study. Using real-time polymerase chain reaction (PCR), the NS5B region of the HCV genome (436 base pair) was amplified, sequenced, and compared with unrelated NS5B sequences.
RESULTS
NS5B sequences were obtained from 200 (89%) cases. Circulating HCV genotypes were 1a (59%), 4d (23%), 3a (11%), 1b (5%), and 2b/c (3%). Phylogenetic analysis revealed 156 (78 …
引用总数
学术搜索中的文章
T van de Laar, O Pybus, S Bruisten, D Brown, M Nelson… - Gastroenterology, 2009