作者
Mariana Sequetin Cunha, Adriana Luchs, Antonio Charlys da Costa, Geovani de Oliveira Ribeiro, Fabiana Cristina Pereira Dos Santos, Juliana Silva Nogueira, Shirley Vasconcelos Komninakis, Robson dos Santos Souza Marinho, Steven S Witkin, Fabiola Villanova, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Elcio Leal, Mauricio Lacerda Nogueira, Paulo César Maiorka
发表日期
2020/5/1
期刊
Acta tropica
卷号
205
页码范围
105401
出版商
Elsevier
简介
In Brazil, flaviviruses have caused massive outbreaks. Surveillance programs designed to monitor virus activity in vectors provides a system for mapping disease distribution and for identifying specific vector species for targeted control. The present study aimed to describe the detection, whole genome characterization and phylogenetic analysis of Ilheus virus (ILHV) and Iguape virus (IGUV) strains obtained from historical mosquito's samples. Twelve isolates of pooled mosquito specimens (inoculated in neonate mouse brain) collected in the state of São Paulo, Brazil, in 1993, 1994 and 1997 were investigated. Viral RNA was extracted and analyzed by qRT-PCR using Flavivirus genus-specific primers. Positive samples were sequenced and underwent phylogenetic analyses. Flavivirus was detected in 50% of the specimens. Positive samples were successfully Sanger sequenced. Three Anopholes cruzii pools …
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MS Cunha, A Luchs, AC da Costa… - Acta tropica, 2020