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Moringa oleifera is a plant with various medicinal benefits. Medicinal properties attribute to the presence of various antioxidants. However, antioxidant activities of Moringa oleifera leaves from different regions of the world have different ranges. This study was designed to evaluate the antioxidant activity of Moringa oleifera grown in Bangladesh. Dried leaves of Moringa oleifera were extracted in methanol. Qualitative and quantitative analyses were done for the presence and content of phytochemicals of Moringa oleifera leaves meathanolic extract (MOLME). Total phenolics and flavonoids contents were determined as 97.33 ± 1.12mg/gm of dry extract as GAE and 32.03 ± 2.53mg/gm of dry extract as CAE, respectively. Antioxidant activities were determined in terms of DPPH, ABTS and lipid peroxidation inhibition assay. IC50 values of DPPH, ABTS and lipid peroxidation inhibition were found 554.47 ± 5.21 μg/ml, 41.33 ± 0.07 μg/ml and 743.14 ± 6.67 μg/ml respectively. Phytochemical contents and antioxidant properties from different regions were compared with the findings of our study. It was observed that, phytoconstituents content and antioxidant potentials of Moringa oleifera vary with different geographical locations. Quality of soil, which is controlled by various factors such as nutrients, water content etc, leads to the variations in phytochemical content and antioxidant properties of MOLME.