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Exercise‐induced fatigue can change motor performance in non‐exercised muscles. The objective was to investigate unilateral elbow flexion (EF) fatigue effects on the maximal voluntary force (MVC) and corticospinal excitability of contralateral non‐exercised biceps brachii (BB). Transcranial magnetic, transmastoid electrical, and brachial plexus electrical stimulation were used to elicit motor evoked potentials (MEP), cervicomedullary motor evoked potentials (CMEP), and compound muscle action potentials in the contralateral non‐exercised BB of 12 participants before and after (i) two bouts of 100‐s unilateral EF (fatigue) or (ii) control. Three stimuli were evoked every 1.5 s during a series of 6‐s isometric EF at 100%, 50%, and 5% of MVC. The non‐exercised EF MVC force, electromyographic activity, and voluntary activation were not significantly different between fatigue and control. Non‐exercised BB MEP and …