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This study examined the mediator effects of the fear of negative evaluation (FNE) on the relation between academic stress and psychological symptoms in a sample of Ghanaian college students (N = 431). A multi-step structural equation modelling (SEM) procedure was used to test the specified mediator model and several fit indices were calculated to assess how well the model fit the data. In addition, path coefficients were calculated to determine whether mediation had been established. The results indicated that the specified model produced a good fit to the data. Moreover, the findings revealed that FNE partially mediated the relation between academic stress and the psychological symptoms of anxiety and depression. Implications for the study’s findings are discussed and recommendations for future research are presented.