作者
Kazumasa Morita, Hiroyuki Sasaki, Mikio Furuse, Shoichiro Tsukita
发表日期
1999/10/4
期刊
The Journal of cell biology
卷号
147
期号
1
页码范围
185-194
出版商
The Rockefeller University Press
简介
Tight junctions (TJs) in endothelial cells are thought to determine vascular permeability. Recently, claudin-1 to -15 were identified as major components of TJ strands. Among these, claudin-5 (also called transmembrane protein deleted in velo-cardio-facial syndrome [TMVCF]) was expressed ubiquitously, even in organs lacking epithelial tissues, suggesting the possible involvement of this claudin species in endothelial TJs. We then obtained a claudin-6–specific polyclonal antibody and a polyclonal antibody that recognized both claudin-5/TMVCF and claudin-6. In the brain and lung, immunofluorescence microscopy with these polyclonal antibodies showed that claudin-5/TMVCF was exclusively concentrated at cell–cell borders of endothelial cells of all segments of blood vessels, but not at those of epithelial cells. Immunoreplica electron microscopy revealed that claudin-5/TMVCF was a component of TJ strands. In …
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K Morita, H Sasaki, M Furuse, S Tsukita - The Journal of cell biology, 1999