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The miniaturized and biocompatible platforms for laser-like emission have been vastly searched in recent times. Therefore, we studied that random laser (RL) features for the dye-doped polymer are affected by the well-known structural differences in the eggshell membrane (ESM). This membrane is composed of three 0.8 μm diameter high-density fiber layers (inner layers of the ESM: IESM) and six 1.1 μm diameter sparse fibers layers (outer layers of the ESM: OESM). The addition of the membrane in 10⁻⁵ M Rhodamine 6G (Rh6G)-doped polymer was performed in such a way that the excitation energy for RL measurements hits the inner ESM layers for one sample and hits the outer ESM layers for the other sample. For comparison purposes, a sample without the membrane was also fabricated with the same Rh6G concentration. RL emissions were obtained for all samples with emission linewidth smaller than 10 …