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Cell-penetrating peptides constitute a promising strategy for the intracellular delivery of therapeutic molecules, such as nucleic acids. The non-covalent approach based on the amphipathic N-TER peptide has shown to be successful in the intracellular delivery of miRNA-27a by inducing its antiadipogenic effect in 3T3-L1 cells. However, information regarding the events taking place at the cellular interface is lacking. Furthermore, information on the uptake process often comes as confusing and contradictive. The main focus of our study is to examine how the peptide interacts with the cell membrane, is this interaction fatal for the cells, or is it just the first step towards the peptide’s internalization into the cells?