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Hydrolytic enzymes have a huge role in the ethanol industry since second generation (cellulosic) agro-industrial wastes have been used as raw materials for these processes. Submerged fermentation for enzymes production is widely used at the moment, but solidstate cultivation could become a cheaper process in the future. With this in mind, the purpose of this work was to compare the two aforementioned techniques by using experimentally obtained data about the fermentation stage in order to compare the two production processes through simulation. Although the experiments showed that more enzymes are being produced by the submerged cultivation method, the comparison of the two processes (submerged and solid-state) through simulation showed that the solid-state technique has better economic parameters. The profitability analysis showed that the solid-state enzyme production could be an economically reasonable solution.